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Our laboratory gathers biologists, physicists and chemists who are interested in the structure, structural dynamics and physical chemistry of membrane proteins, either in vivo within a lipid bilayer, or in vitro after solubilization and stabilization by classical or alternative surfactants such as amphipols.<\/p>\n<\/div>\n<\/div>\n<\/div>\n
\u2192 Theme 1. Bruno Miroux, Karine Moncoq, Daniel Picot and Francesca Zito, Manuela Zoonens : Structural bioenergetics : It combines i) a methodological approach to the crystallography of membrane proteins and ii) a study of electron transfer chain in photosynthesis and respiration, centred on the linchpin joint complex: the complex Rieske\/cytochrome b. It includes studies of the possible source of ROS production and the organization of the different complexes of the electron transfer chain in supercomplexes.<\/p>\n Mitochondrial bioenergetics: it\u00a0focuses on the structure-function relationships of mitochondrial uncoupling proteins and other carriers in connection with mitochondrial metabolism. The role of fatty acid in transport mechanisms will be more specifically investigated.<\/p>\n <\/p>\n \u2192\u00a0Theme 2<\/strong>. Val\u00e9rie Biou and Laurent Catoire : We use NMR and X-ray crystallography combined with other biophysical methods to study the various actors involved in signal transduction by G protein-coupled receptors: ligands, receptors and intracellular effectors. We are presently focussing on lipid signalling (leukotriene receptors) and the Hedgehog signalling pathway. Obtaining various proteins necessarily implies an important activity of molecular biology and biochemistry. Downstream biophysical studies also include methodological development.<\/p>\n <\/p>\n \u2192\u00a0Theme 3<\/strong>. Philippe Delepelaire and Dror Warschawski : Through various methods, ranging from microbial physiology to solid state NMR, we study model systems recognition phenomena and substrate transport across the bacterial envelope, and related signaling pathways.<\/p>\n Within this framework, we study the transport of porphyrins across the bacterial envelope, and the bacterial response to mechanical stress. Since solid-state NMR allows the study of membrane proteins inserted into membranes, we favor the study of samples either in model lipid membranes, or directly in native bacterial membranes.<\/p>\n <\/p>\n \u2192\u00a0Theme\u00a04,<\/strong>\u00a0transversal. Fabrice Giusti, Bruno Miroux, Jean-Luc Popot, Dror Warschawski, Francesca Zito and Manuela Zoonens : This theme contains methodological developments necessary to the other three themes : (2) Chemical ligands and surfactants<\/strong>\u00a0: this module includes :<\/p>\n a) the synthesis and validation of amphipols functionalized so-called second generation (deuteration, biotin, polyhistidine, imidazole, oligonucleotides, different fluorescent labels…) for stabilization and purification and vectorization of membrane proteins<\/p>\n b) the synthesis of photoactivatable ligands and substrates for structural and functional studies<\/p>\n<\/div>\n<\/div>\n<\/div>\n Responsable de l’unit\u00e9 : Bruno MIROUX<\/p>\n Energy coupling & supramolecular organization of respiratory chain complexes<\/strong>\u00a0:<\/p>\n *Respiratory chain complexes : *Uncoupling protein biochemistry : Technical support Molecular Signalisation Pathway of GPGRs<\/strong>\u00a0: *Study of Signal Transduction by GPCRs *GPCR Cristallization Technical support Transport and Membrane Dynamics in Bacteria\u00a0<\/strong>: *Membrane biogenesis in E. coli *Solid state NMR of membranes and membrane proteins *Efflux pumps in bacteria Technical support Institut de Biologie Physico-Chimique (IBPC)<\/p>\n 13, rue Pierre et Marie Curie<\/p>\n 75252 PARIS CEDEX 05<\/p>\n<\/div>\n<\/div>\n<\/div>\n 75005<\/span>\u00a0PARIS<\/span><\/div>\n<\/div>\n [\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row][et_pb_column type=\u00a0\u00bb1_2″][\/et_pb_column][et_pb_column type=\u00a0\u00bb1_2″][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":" LBPC-PM \u00a0 Tutelles\u00a0> Universit\u00e9 Paris Diderot – Paris 7 \u00a0 UFR de rattachement\u00a0> Sciences du Vivant \u00a0 Label unit\u00e9 > UMR 7099 \u00a0 Partenaires\u00a0> CNRS \u00a0 Pr\u00e9sentation Our laboratory gathers biologists, physicists and chemists who are interested in the structure, structural dynamics and physical chemistry of membrane proteins, either in vivo within a lipid… Lire la suite Laboratoire de Biologie Physico-Chimique des Prot\u00e9ines Membranaires<\/span><\/a><\/p>\n","protected":false},"author":69,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-1588","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/wordpress-test.app.u-pariscite.fr\/sdv\/wp-json\/wp\/v2\/pages\/1588","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wordpress-test.app.u-pariscite.fr\/sdv\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wordpress-test.app.u-pariscite.fr\/sdv\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wordpress-test.app.u-pariscite.fr\/sdv\/wp-json\/wp\/v2\/users\/69"}],"replies":[{"embeddable":true,"href":"https:\/\/wordpress-test.app.u-pariscite.fr\/sdv\/wp-json\/wp\/v2\/comments?post=1588"}],"version-history":[{"count":2,"href":"https:\/\/wordpress-test.app.u-pariscite.fr\/sdv\/wp-json\/wp\/v2\/pages\/1588\/revisions"}],"predecessor-version":[{"id":1968,"href":"https:\/\/wordpress-test.app.u-pariscite.fr\/sdv\/wp-json\/wp\/v2\/pages\/1588\/revisions\/1968"}],"wp:attachment":[{"href":"https:\/\/wordpress-test.app.u-pariscite.fr\/sdv\/wp-json\/wp\/v2\/media?parent=1588"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
Energy coupling and supramolecular organization of respiratory complexes<\/em><\/p>\n
Molecular signalling of GPCRs<\/em><\/p>\n
Transport and membrane dynamics in bacteria<\/em><\/p>\n
A toolbox for the chemistry of membrane proteins<\/em><\/p>\n
(1)<\/strong>\u00a0Production of membrane proteins<\/strong>\u00a0: approaches to bacterial molecular genetics and chemistry of surfactants are being developed for membrane protein production in vivo and in vitro with particular attention to strategies for isotopic labeling.<\/p>\nEquipes de recherche<\/h3>\n
Daniel PICOT (DR2 CNRS)
Francesca ZITO (CR1 CNRS)
Nathalie SISATTANA (PhD student Labex Dynamo)
Nisha GOPAL (M2 Fullbright fellowship)<\/p>\n
Bruno MIROUX (DR2 INSERM)
Karine MONCOQ (MCU Paris Diderot)
Manuela ZOONENS (CR1 CNRS)
Mathilde PIEL (PhD student MRT)<\/p>\n
Oana ILIOAIA (IE2 CNRS)
Sandrine MASSCHELEYN (AI CNRS)
Elodie POINT-BONNET (TCS CNRS)<\/p>\n
*HEDGEHOG Pathway
Val\u00e9rie BIOU (CR1 CNRS)
Sta\u00eblle MAKAMTE (PhD IDEX SPC)
Adlen GRINE (M2)<\/p>\n
Laurent J. CATOIRE (CR1 CNRS)
Marina CASIRAGHI (Post-doc labex Dynamo)<\/p>\n
Karine MONCOQ (MCU Paris Diderot)<\/p>\n
Annick PAQUELIN (TCE CNRS)
Elodie POINT-BONNET (TCS CNRS)<\/p>\n
*Biochemistry of Iron Transport in Haemophilus
Philippe DELEPELAIRE (DR2 CNRS)
Marc UZAN (DR2 CNRS)
Yvette NTSOGO (Post-doc labex Dynamo)<\/p>\n
Bruno MIROUX (DR2 INSERM)
Federica ANGIUS (PhD student labex Dynamo)<\/p>\n
Dror Warschawski (CR1 CNRS)<\/p>\n
Martin PICARD (CR1 CNRS)
Hager SOUABNI (Post-doc FRM fellowship)
Dhenesh PUVANENDRAN (PhD student MRT)<\/p>\n
Oana ILIOAIA (IE2 CNRS)
Annick PAQUELIN (TCE CNRS)<\/p>\n<\/div>\n<\/div>\n<\/div>\nAUTRES CONTACTS<\/h3>\n
Localisation<\/h3>\n