[et_pb_section bb_built=\u00a0\u00bb1″ next_background_color=\u00a0\u00bb#000000″][et_pb_row][et_pb_column type=\u00a0\u00bb4_4″][et_pb_post_title _builder_version=\u00a0\u00bb3.22.1″ meta=\u00a0\u00bboff\u00a0\u00bb featured_image=\u00a0\u00bboff\u00a0\u00bb text_shadow_horizontal_length=\u00a0\u00bbtext_shadow_style,%91object Object%93″ text_shadow_vertical_length=\u00a0\u00bbtext_shadow_style,%91object Object%93″ text_shadow_blur_strength=\u00a0\u00bbtext_shadow_style,%91object Object%93″ title_text_shadow_horizontal_length=\u00a0\u00bbtitle_text_shadow_style,%91object Object%93″ title_text_shadow_vertical_length=\u00a0\u00bbtitle_text_shadow_style,%91object Object%93″ title_text_shadow_blur_strength=\u00a0\u00bbtitle_text_shadow_style,%91object Object%93″ meta_text_shadow_horizontal_length=\u00a0\u00bbmeta_text_shadow_style,%91object Object%93″ meta_text_shadow_vertical_length=\u00a0\u00bbmeta_text_shadow_style,%91object Object%93″ meta_text_shadow_blur_strength=\u00a0\u00bbmeta_text_shadow_style,%91object Object%93″ z_index_tablet=\u00a0\u00bb500″ \/][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section bb_built=\u00a0\u00bb1″ inner_width=\u00a0\u00bbauto\u00a0\u00bb inner_max_width=\u00a0\u00bbnone\u00a0\u00bb prev_background_color=\u00a0\u00bb#000000″][et_pb_row][et_pb_column type=\u00a0\u00bb4_4″][et_pb_image _builder_version=\u00a0\u00bb3.22.1″ src=\u00a0\u00bbhttps:\/\/wordpress-test.app.u-pariscite.fr\/sdv\/wp-content\/uploads\/sites\/4\/2020\/02\/Physio-c\u00e9r\u00e9brale.jpg\u00a0\u00bb z_index_tablet=\u00a0\u00bb500″ \/][\/et_pb_column][\/et_pb_row][et_pb_row][et_pb_column type=\u00a0\u00bb1_3″][\/et_pb_column][et_pb_column type=\u00a0\u00bb1_3″][\/et_pb_column][et_pb_column type=\u00a0\u00bb1_3″][\/et_pb_column][\/et_pb_row][et_pb_row][et_pb_column type=\u00a0\u00bb4_4″][et_pb_text _builder_version=\u00a0\u00bb3.22.1″ module_id=\u00a0\u00bbcontenu\u00a0\u00bb text_text_shadow_horizontal_length=\u00a0\u00bbtext_text_shadow_style,%91object Object%93″ text_text_shadow_vertical_length=\u00a0\u00bbtext_text_shadow_style,%91object Object%93″ text_text_shadow_blur_strength=\u00a0\u00bbtext_text_shadow_style,%91object Object%93″ link_text_shadow_horizontal_length=\u00a0\u00bblink_text_shadow_style,%91object Object%93″ link_text_shadow_vertical_length=\u00a0\u00bblink_text_shadow_style,%91object Object%93″ link_text_shadow_blur_strength=\u00a0\u00bblink_text_shadow_style,%91object Object%93″ ul_text_shadow_horizontal_length=\u00a0\u00bbul_text_shadow_style,%91object Object%93″ ul_text_shadow_vertical_length=\u00a0\u00bbul_text_shadow_style,%91object Object%93″ ul_text_shadow_blur_strength=\u00a0\u00bbul_text_shadow_style,%91object Object%93″ ol_text_shadow_horizontal_length=\u00a0\u00bbol_text_shadow_style,%91object Object%93″ ol_text_shadow_vertical_length=\u00a0\u00bbol_text_shadow_style,%91object Object%93″ ol_text_shadow_blur_strength=\u00a0\u00bbol_text_shadow_style,%91object Object%93″ quote_text_shadow_horizontal_length=\u00a0\u00bbquote_text_shadow_style,%91object Object%93″ quote_text_shadow_vertical_length=\u00a0\u00bbquote_text_shadow_style,%91object Object%93″ quote_text_shadow_blur_strength=\u00a0\u00bbquote_text_shadow_style,%91object Object%93″ header_text_shadow_horizontal_length=\u00a0\u00bbheader_text_shadow_style,%91object Object%93″ header_text_shadow_vertical_length=\u00a0\u00bbheader_text_shadow_style,%91object Object%93″ header_text_shadow_blur_strength=\u00a0\u00bbheader_text_shadow_style,%91object Object%93″ header_2_text_shadow_horizontal_length=\u00a0\u00bbheader_2_text_shadow_style,%91object Object%93″ header_2_text_shadow_vertical_length=\u00a0\u00bbheader_2_text_shadow_style,%91object Object%93″ header_2_text_shadow_blur_strength=\u00a0\u00bbheader_2_text_shadow_style,%91object Object%93″ header_3_text_shadow_horizontal_length=\u00a0\u00bbheader_3_text_shadow_style,%91object Object%93″ header_3_text_shadow_vertical_length=\u00a0\u00bbheader_3_text_shadow_style,%91object Object%93″ header_3_text_shadow_blur_strength=\u00a0\u00bbheader_3_text_shadow_style,%91object Object%93″ header_4_text_shadow_horizontal_length=\u00a0\u00bbheader_4_text_shadow_style,%91object Object%93″ header_4_text_shadow_vertical_length=\u00a0\u00bbheader_4_text_shadow_style,%91object Object%93″ header_4_text_shadow_blur_strength=\u00a0\u00bbheader_4_text_shadow_style,%91object Object%93″ header_5_text_shadow_horizontal_length=\u00a0\u00bbheader_5_text_shadow_style,%91object Object%93″ header_5_text_shadow_vertical_length=\u00a0\u00bbheader_5_text_shadow_style,%91object Object%93″ header_5_text_shadow_blur_strength=\u00a0\u00bbheader_5_text_shadow_style,%91object Object%93″ header_6_text_shadow_horizontal_length=\u00a0\u00bbheader_6_text_shadow_style,%91object Object%93″ header_6_text_shadow_vertical_length=\u00a0\u00bbheader_6_text_shadow_style,%91object Object%93″ header_6_text_shadow_blur_strength=\u00a0\u00bbheader_6_text_shadow_style,%91object Object%93″ z_index_tablet=\u00a0\u00bb500″]<\/p>\n
La th\u00e9matique de l’UMR est centr\u00e9e sur l’\u00e9laboration et la transmission des signaux d’information nerveuse dans le cerveau.<\/strong><\/p>\n Cette information nerveuse est \u00e9tudi\u00e9e aux niveaux sous-cellulaire, cellulaire et multicellulaire (petits r\u00e9seaux neuronaux).<\/p>\n Ces \u00e9tudes font appel \u00e0 des techniques \u00e9lectrophysiologiques pour l’enregistrement des signaux \u00e9lectriques, \u00e0 l\u2019analyse de processus nerveux par des m\u00e9thodes performantes d\u2019imagerie calcique, au marquage immunologique de prot\u00e9ines sp\u00e9cifiques en microscopie optique et \u00e9lectronique, et aux m\u00e9thodes de biologie mol\u00e9culaire pour aider \u00e0 la dissection du r\u00f4le fonctionnel de ces prot\u00e9ines.<\/p>\n<\/div>\n<\/div>\n<\/div>\n Our work in the last decade has aimed at unraveling molecular and cellular aspects of synaptic transmission and intracellular calcium homeostasis in the mammalian cerebellar cortex using electrophysiology and optical techniques approaches. One aspect of this work is the study of the molecular profile and spatial localization of ion channels and transmitter-gated receptors in GABA-releasing neurons in cerebellar slices with focus on molecules governing responses to the excitatory neurotransmitter glutamate.<\/p>\n A second axis of the work is the analysis of calcium signaling in the various compartments of cerebellar neurons, with particular emphasis on activity-dependent calcium signals generated in the axon. Using digital fluorescence imaging and 2 photon laser scanning microscopy, we study the temporal and spatial properties of calcium signals in axonal varicosities of both, interneurons and Purkinje cells. We are interested in how voltage-gated calcium channels, calcium binding proteins and intracellular calcium stores shape calcium signals and subsequent neurotransmitter release. Interactions between metabotropic glutamate receptors (mGluRs) and calcium signaling have also been a recent theme of our studies.<\/p>\n In the past few years, our team has expanded activities from the in vitro slice preparation and we are now also working with an in vivo system. Using 2 photon laser scanning microscopy in anesthetized mice, we first characterized the properties of somatic calcium transients linked with action potentials in molecular layer interneurons loaded with organic calcium indicators. More recently, we are performing in vivo imaging in interneurons from mice expressing various varieties of the genetically-encoded calcium proteins GCaMP3 and GCaMP5.<\/p>\n This recent push to work in vivo gives us the possibility of studying various molecular elements of the cerebellar neurons in the intact animal. For instance, because of our previous work in vitro, showing that mGluRs have a powerful influence on the spiking pattern and intracellular calcium signaling of molecular layer interneurons, we are interested in dissecting the contribution of mGluRs to calcium signals in vivo. We are also interested in probing for the presence of functional presynaptic receptors in the intact animaI and in assessing their role in neuronal integration.<\/p>\n An important goal for the future years will be to link the optical monitoring of neuronal activity with specific sensorimotor tasks in the awake animal. Establishing new procedures to study cerebellar circuits in vivo by the incorporation of new techniques such as local photostimulation or development of novel genetic tools to apply viral mediated expression strategies with appropriate viral vectors is a central goal of our work.<\/p>\n<\/div>\n<\/div>\n<\/div>\n Alain Marty (Directeur de l’unit\u00e9) : Synapses gabaergiques du cervelet<\/p>\n Isabel Llano et\u00a0<\/strong>Thibault Collin (responsables)\u00a0<\/strong>: Imagerie calcique de neurones centraux (en partenariat avec l’UFR SDV de Paris Diderot)<\/strong><\/p>\n Philippe Ascher : R\u00e9cepteurs du glutamate<\/p>\n David Ogden : D\u00e9veloppement de la photolib\u00e9ration de compos\u00e9s cag\u00e9s pour la neurophysiologie et l’\u00e9tude des r\u00e9cepteurs du glutamate<\/p>\n David Digregorio\u00a0 : Examen de connexions synaptiques individuelles avec des m\u00e9thodes optiques<\/p>\n Christophe Pouzat : Statistiques computationnelles pour l’analyse de donn\u00e9es neurophysiologiques<\/p>\n<\/div>\n<\/div>\n<\/div>\n UMR 8118 75006<\/span>\u00a0PARIS<\/span><\/div>\n<\/div>\n [\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row][et_pb_column type=\u00a0\u00bb1_2″][\/et_pb_column][et_pb_column type=\u00a0\u00bb1_2″][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":" Tutelles\u00a0> Universit\u00e9 Paris Descartes \u00a0 UFR de rattachement\u00a0> Sciences du Vivant \u00a0 Label unit\u00e9 > UMR 8118 \u00a0 Partenaires\u00a0> CNRS \u00a0 Pr\u00e9sentation La th\u00e9matique de l’UMR est centr\u00e9e sur l’\u00e9laboration et la transmission des signaux d’information nerveuse dans le cerveau. Cette information nerveuse est \u00e9tudi\u00e9e aux niveaux sous-cellulaire, cellulaire et multicellulaire (petits r\u00e9seaux neuronaux).… Lire la suite Physiologie C\u00e9r\u00e9brale<\/span><\/a><\/p>\n","protected":false},"author":69,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-1579","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/wordpress-test.app.u-pariscite.fr\/sdv\/wp-json\/wp\/v2\/pages\/1579","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wordpress-test.app.u-pariscite.fr\/sdv\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wordpress-test.app.u-pariscite.fr\/sdv\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wordpress-test.app.u-pariscite.fr\/sdv\/wp-json\/wp\/v2\/users\/69"}],"replies":[{"embeddable":true,"href":"https:\/\/wordpress-test.app.u-pariscite.fr\/sdv\/wp-json\/wp\/v2\/comments?post=1579"}],"version-history":[{"count":2,"href":"https:\/\/wordpress-test.app.u-pariscite.fr\/sdv\/wp-json\/wp\/v2\/pages\/1579\/revisions"}],"predecessor-version":[{"id":1581,"href":"https:\/\/wordpress-test.app.u-pariscite.fr\/sdv\/wp-json\/wp\/v2\/pages\/1579\/revisions\/1581"}],"wp:attachment":[{"href":"https:\/\/wordpress-test.app.u-pariscite.fr\/sdv\/wp-json\/wp\/v2\/media?parent=1579"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}Th\u00e8mes de recherche<\/h3>\n
Equipes de recherche<\/h3>\n
AUTRES CONTACTS<\/h3>\n
Universit\u00e9 Paris Descartes
45 rue des Saints-P\u00e8res
75270 Paris cedex 06<\/p>\n<\/div>\n<\/div>\n<\/div>\nLocalisation<\/h3>\n